题目一：Chemical Nanolithography
题目二：In-situ studies of marine fouling
时 间：2011年11月14日(星期一) 9:50
地 点：玉泉校区教七306多媒体教室
题目三：Measuring the distribution of cell adhesion strength
时 间：2011年11月14日(星期一) 15:00
地 点：高分子楼二楼学术报告厅 (228房间)
邀请人：计 剑 教授
Michael Grunze教授简介：
Prof. Dr. Michael Grunze is Chair of the Applied Physical Chemistry Research Group at Heidelberg University’s Institute of Physical Chemistry. He received his diploma in chemistry in 1972 and his Ph.D. in physical chemistry in 1974 from the Free University of Berlin. From 1984-1988, he was Full Professor of Physics and Adjunct Professor of Chemistry at the University of Maine in Orono, from 1985-1987, Director of the Laboratory for Surface Science and Technology at the University of Maine, and from 2003-2007, Codirector of the Institute of Molecular Biophysics at the Jackson Laboratory in Bar Harbor, Maine. During his tenure in Maine he started to work on polymer surfaces, which led into the ongoing investigation of ultrathin organic films and self-assembled monolayers in his group in Heidelberg. His present research focuses on the preparation and characterization of organic films, including their applications, and the development of spectroscopic methods for in situ and in vivo investigations of biointerfaces and cells. Michael Grunze received several awards and serves as the chairman of the Scientific Advising Board of the Max-Planck-Institute of Colloid and Interface Science in Golm, Germany. He is a member of the Scientific Advisory Board of the European Synchrotron Source Radiation Facility (ESRF) and several research institutes in Europe, and is scientific director of CeloNova, Newnan, Georgia. Georgia. He became a member of AVS in 1981 and is editor-in-chief of Biointerphases. Prof. Grunze has published more than 400 scientific papers in international journals, including Science, Nature, J. Am. Chem. Soc., Angew. Chem. Int. Ed., Adv. Mater., Phys. Rev. Lett., ACS Nano, etc.
报告内容：
Title 1. Chemical Nanolithography
Abstract:
In this talk the advances in the fabrication of chemical and morphological nano-patterns and gradients by electron beam chemical lithography (EBCL) -or ”Chemical Nanolithography” -with monomolecular films are discussed. We will show that “Chemical Nanolithography” is an easy and convenient method to create multiphase organic, polymeric or biological surface nanostructures and gradients using electron beam writing or stencil masks, combined with chemical surface modifications. Aliphatic monolayers are more radiation sensitive than aromatic films and hence require a smaller irradiation dose for patterning. Specific examples given in this talk refer to irradiation-promoting exchange reaction (IPER) lithography, electron beam activation lithography (EBAL), and direct writing chemical lithography (DWCL). However, chemical patterns that can subsequently be developed into 3D polymer brush architectures can also be made by carbon templating from the gas phase without a pre-adsorbed organic monolayer.
Title2. In-situ studies of marine fouling
Abstract:
The settlement and colonization of marine organisms on submerged man-made surfaces is a major economic and environmental problem for marine industries. The historical paradigm is the use of toxic materials to kill fouling organisms. However, since 2008 the use of biocides (in particular tributyltin) is restricted and thus environmentally benign but effective surface coatings are required. Here I describe how in situ in line optical holography observations of the surface exploration behavior of spores can predict within 1-2 minutes the attractiveness of a surface coating for biofouling. We prepared different model surfaces with a systematic variation of surface properties to distinguish the effects of surface energy, surface chemical composition, hydration, charge and topography and morphology on surface colonization by marine organisms.
Title 3. Measuring the distribution of cell adhesion strength
Abstract:
The adhesion strength of cells depends on the properties of the surface they attach to. Varying the surface properties can trigger different cellular responses such as differentiation. In order to study cell adhesion quantitatively, we developed a microfluidic shear force assay, which allows the variation of applied shear stress by five orders of magnitude. With this device we can determine the critical shear stress that is necessary to remove 50% of the adherent cells. As an application, we investigated the adhesion strength of functionalized beads, cells, and bacteria on a selection of SAM surfaces, including a series of oligo(ethylene glycol) (OEG)containing self-assembled monolayers (SAMs). By varying the number of ethylene oxide units, the hydration properties of the monolayers are changed. We found that cell adhesion strength for mammalian fibroblasts decreases if the hydration of the surface is increased. As the cell spreading area changes with the substrate properties, the adhesion strength per unit area was additionally determined.
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